On-line urea kinetics in haemodiafiltration
Open Access
- 1 June 1996
- journal article
- Published by Oxford University Press (OUP) in Nephrology Dialysis Transplantation
- Vol. 11 (6) , 1084-1092
- https://doi.org/10.1093/ndt/11.6.1084
Abstract
Calculation of Kt/V and assessment of nutrition have so far been dependent upon off-line urea measurements of blood or dialysate samples. Here we describe a biosensor for on-line urea measurement during haemodiafiltration. The biosensor consisted of a cartridge containing covalently linked urease placed between two conductivity cells. The biosensor was placed on the outlet line of a haemofilter in series with a dialyser in order to obtain an aliquot of plasma ultrafiltrate for on-line measurement of urea. Urea nitrogen concentrations were highly correlated to the difference (Δ) in conductivity measured by the two conductivity cells both in aqueous solutions (in-vitro studies, y=−6.676+32.12x, R2=0.998, Pex-vivo studies, y=−6.7+32.01x, R2=0.98, P2=0.99, R2=0.97 respectively). These results indicate that on-line, real-time analysis of urea kinetics may provide information on delivery of adequate dialysis in high-efficiency techniques.Keywords
This publication has 0 references indexed in Scilit: