Circular dichroism determination of class I MHC‐peptide equilibrium dissociation constants
Open Access
- 1 August 1997
- journal article
- for the-record
- Published by Wiley in Protein Science
- Vol. 6 (8) , 1771-1773
- https://doi.org/10.1002/pro.5560060819
Abstract
Class I major histocompatibility complex (MHC) molecules bind peptides derived from degraded proteins for display to T cells of the immune system. Peptides bind to MHC proteins with varying affinities, depending upon their sequence and length. We demonstrate that the thermal stability of the MHC-peptide complex depends directly on peptide binding affinity. We use this correlation to develop a convenient method to determine peptide dissociation constants by measuring MHC-peptide complex stability using thermal denaturation profiles monitored by circular dichroism.Keywords
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