Altered Gene Expression Levels of Matrix Metalloproteinases and Their Inhibitors in Periodontitis‐Affected Gingival Tissue

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Abstract
Background: The balance between the degradation and synthesis of extracellular matrix determines periodontal attachment levels and alveolar bone matrix concentration in periodontal diseases. Matrix metalloproteinases (MMPs) are known to degrade periodontal ligamental attachment and bone matrix proteins. The purpose of this study was to examine the effect of different expression levels of MMPs and their inhibitors, the tissue inhibitors of matrix metalloproteinases (TIMPs), in periodontitis.Methods: Sixteen inflamed gingival tissue samples from subjects with generalized chronic periodontitis and 14 control tissue samples from systemically and periodontally healthy subjects were evaluated. The total RNA was extracted, and the transcript levels for MMP‐1, −3, −9, and −13 and TIMP‐1, −2, −3, and −4 relative to β‐actin were determined by quantitative real‐time reverse transcription‐polymerase chain reaction.Results: Gene transcript levels for MMP‐1 and TIMP‐4 were significantly higher in periodontitis‐affected gingival tissues (P P P P P Conclusions: Upregulated MMP expression and an increased MMP/TIMP ratio indicate that a potential imbalance between degradation and synthesis of extracellular matrix persists in periodontitis‐affected gingival tissues. This process may be responsible for increased tissue breakdown in periodontitis.

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