Cultivation of Two Species of Neorickettsia in Canine Monocytes

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Abstract
Two neorickettsial canine pathogens were isolated from infected dogs and maintained in cultivated canine monocytes. The leukocytic response to infection was determined by examination of coverslips stained by May-Grünwald and fluorescent antibody techniques, by electron microscopic evaluation, and by electron microscopy of histochemically stained infected cells. No differences between the two neorickettsial agents were found when grown in tissue culture. The morphologic response of infected monocytes was not remarkable until organisms were numerous. Organisms regarded as viable were found in cytoplasmic vacuoles lacking peroxidase or acid phosphatase reaction product. Degraded organisms were found only in vacuoles with reaction product. Neorickettsia-infected macrophages were found to be a useful model for examination of interactions between macrophages and parasites.

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