Computer‐aided assignment of the 1H‐NMR spectrum of the viral‐protein‐genome‐linked polypeptide from cowpea mosaic virus
- 1 June 1990
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 190 (3) , 583-591
- https://doi.org/10.1111/j.1432-1033.1990.tb15613.x
Abstract
The 1H‐NMR spectrum of the viral‐protein‐genome‐linked (VPg) polypeptide from cowpea mosaic virus, has been interpreted via application of 2‐dimensional (2D) NMR techniques. The interpretation of the data was performed by a computer program called ‘PROSPECT’ (PROtein SPECTra), which detects the cross‐peak patterns of the amino acid residues in the spectra, assigns these patterns to amino acid types, and finally performs the sequential assignments using the well‐known ‘sequential walks’ obtained from the NOE spectrum.Due to the severe overlap of resonances in the NMR spectrum of the VPg polypeptide, several ways of performing these walks existed. The program detected six alternatives for the sequential assignments of backbone NαH‐CβH‐CβH moieties.Keywords
This publication has 12 references indexed in Scilit:
- PROSPECT, a program for automated interpretation of 2D NMR spectra of proteinsJournal of Magnetic Resonance (1969), 1990
- Toward automatic assignment of protein 1H NMR spectraJournal of Magnetic Resonance (1969), 1989
- Programs for computer-assisted sequential assignment of proteinsJournal of Magnetic Resonance (1969), 1989
- Automated elucidation of J connectivities in 1H NMR spectraJournal of Magnetic Resonance (1969), 1989
- Sequential resonance assignments as a basis for the determination of a three-dimensional structure of protein E-L30 of Escherichia coliJournal of Molecular Biology, 1986
- Residue-specific assignments of resonances in the 1H nuclear magnetic resonance spectrum of ribosomal protein E-L30 by systematic application of two-dimensional fourier transform nuclear magnetic resonance methodsJournal of Molecular Biology, 1986
- Computer aided evaluation of two-dimensional NMR spectra of proteinsBiochemical and Biophysical Research Communications, 1984
- Application of phase sensitive two-dimensional correlated spectroscopy (COSY) for measurements of 1H-1H spin-spin coupling constants in proteinsBiochemical and Biophysical Research Communications, 1983
- Sequential resonance assignments as a basis for determination of spatial protein structures by high resolution proton nuclear magnetic resonanceJournal of Molecular Biology, 1982
- Quadrature fourier NMR detection: Simple multiplex for dual detection and discussionJournal of Magnetic Resonance (1969), 1975