Early detection of influenza virus in cell culture by means of immunofluorescence

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Abstract
An indirect fluorescent antibody technique using human serum for the rapid detection of influenza A virus in monkey kidney cell cultures is described. Influenza A2 virus was isolated from 38 of 88 nose/throat swabs received from patients with suspected influenza during the winter of 1971-72.Thirty-two strains isolated in monkey kidney cell cultures were identified by immunofluorescence on the day haemadsorption was observed, 22 of them within a week of receipt of the specimen. Human antiserum was found to be very satisfactory for this purpose.