Tumor promoters induce the synthesis of a secreted glycoprotein in mouse skin and cultured primary mouse epidermal cells
- 1 January 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in Carcinogenesis: Integrative Cancer Research
- Vol. 2 (10) , 971-976
- https://doi.org/10.1093/carcin/2.10.971
Abstract
The effect of tumor promoters and anti-promoters on the synthesis and secretion of a 35, 000 mol wt transformation-dependent secreted glycoprotein (MEP) by primary mouse epidermal cells has been investigated. MEP is synthesized and secreted at low levels by cultured epidermal basal cells. The phorbol ester 12-O-tetradecanoyl phorbol-13-acetate (TPA) induces a 3-fold increase in the synthesis of MEP as determined by quantitative immunoprecipitation from pulse-labeled cell extracts. This increase is maximal 6 h after treatment and parallels an increase in secretion of MEP measured by appearance of radiolabeled protein in the medium or by radioimmunoassay of culture super-natants. An increase in MEP synthesis is also observed in the skin of mice exposed to TPA. The phorbol ester antagonists retinoic acid, lidocaine and fluocinolone acetonide were examined to determine their effect on the synthesis and secretion of MEP. Of these agents, only fluocinolone acetonide affects MEP levels by suppressing the basal level of MEP synthesis but not its relative induction by TPA. These data suggest that the modifying effects of these three agents on tumor promotion are not mediated via an alteration in MEP synthesis or secretion and that an increase in MEP synthesis per se is not sufficient for promotion.This publication has 15 references indexed in Scilit:
- Tumor promoters and epidermal growth factor stimulate anchorage-independent growth of adenovirus-transformed rat embryo cellsCell, 1979
- Biologically active phorbol esters specifically alter affinity of epidermal growth factor membrane receptorsNature, 1979
- Effect of tumor promoters, protease inhibitors, and repair processes on x-ray-induced sister chromatid exchanges in mouse cells.Proceedings of the National Academy of Sciences, 1979
- Tumor promoter induces sister chromatid exchanges: relevance to mechanisms of carcinogenesis.Proceedings of the National Academy of Sciences, 1978
- Plasminogen activator in chick embryo muscle cells: Induction of enzyme by RSV, PMA and retinoic acidCell, 1978
- Tumor-Promoting Phorbol Esters Inhibit Binding of Epidermal Growth Factor to Cellular ReceptorsScience, 1978
- Transformation-dependent secretion of a low molecular weight protein by murine fibroblasts.Proceedings of the National Academy of Sciences, 1978
- Effects of Tumor Promoters and Steroidal Anti-inflammatory Agents on Skin of Newborn Mice In Vivo and In Vitro2JNCI Journal of the National Cancer Institute, 1978
- Dissociation of tumor promoter-stimulated ornithine decarboxylase activity and DNA synthesis in mouse epidermis in vivo and in vitro by fluocinolone acetonide, a tumor-promotion inhibitor.Proceedings of the National Academy of Sciences, 1977
- The Synthesis Of Specific Proteins In Adult Mouse Epidermis During Phases Of Proliferation And Differentiation Induced By The Tumor Promoter Tpa, And In Basal And Differentiating Layers Of Neonatal Mouse EpidermisJournal of Investigative Dermatology, 1976