Studies in Vitro on Cellular Physiology

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Abstract
Cellular suspensions were prepared from thymus, spleen, bone marrow and testis of rabbits and from leucocytes of normal and leukemic blood of men. The suspensions were irradiated with doses of X-rays varying between 20 and 5000 r/air (Factors: 250 kv, 10 ma., 50 cm. distance, 1 mm, Al filter,120 r/min., HVL 0.7 mm. Cu). After exposure the suspensions were incubated at 37[degree]C for 1-7 days. The irradiated and non-irradiated control suspensions were examined at periodic intervals by the method of unstained cell counts (resistance to eosine) and by stained smears. Exposure of thymic and splenic suspensions to 1000 r caused no perceptible change in the number of eosine resistant (i.e., viable) cells in the first 3 hrs. of incubation but produced a relatively rapid decrease in the unstained cell counts after a short latent period. Irradiated leucocytes from normal blood and from lymphatic leukemic blood had shorter survival periods than non-irradiated cells. Irradiation and incubation of suspensions from bone marrow from myelogenous leukemic blood and from the testicle had no effect on the number of eosine resistant or viable cells when compared to non-irradiated suspensions.