Identification of a novel member of yeast mitochondrial Hsp70‐associated motor and chaperone proteins that facilitates protein translocation across the inner membrane
- 21 December 2004
- journal article
- Published by Wiley in FEBS Letters
- Vol. 579 (2) , 507-511
- https://doi.org/10.1016/j.febslet.2004.12.018
Abstract
Here, we report the identification of yeast 15-kD Tim15/Zim17, a new member of mitochondrial Hsp70 (mtHsp70)-associated motor and chaperone (MMC) proteins. The 15-kD MMC protein is a peripheral inner membrane protein with a zinc-finger motif. Depletion of the 15-kD protein led to impaired import of presequence-containing proteins into the matrix in vivo and in vitro. Overexpression of the 15-kD protein rescued the functional defects of mtHsp70 in ssc1-3 cells, and a fusion protein containing the 15-kD protein physically interacts with purified mtHsp70. Tim15/Zim17 therefore cooperates with mtHsp70 to facilitate import of presequence-containing proteins into the matrix.Keywords
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