An improved method for isolating cardiac myocytes useful for patch-clamp studies.
- 1 January 1990
- journal article
- research article
- Published by Physiological Society of Japan in The Japanese Journal of Physiology
- Vol. 40 (1) , 157-163
- https://doi.org/10.2170/jjphysiol.40.157
Abstract
An improved method for isolating cardiac myocytes is described. This method consists of the initial dispersion of the myocytes with a new type of collagenase and the following treatment of the cells with a non-specific protease. This method gives more than 80% rod-shaped, viable, and quiescent cells, which are suitable for biochemical as well as electrophysiological experiments. Formation of a G.OMEGA. seal and subsequent whole-cell recording is easily performed using these cells.Keywords
This publication has 7 references indexed in Scilit:
- Rapid β-Adrenergic Modulation of Cardiac Calcium Channel Currents by a Fast G Protein PathwayScience, 1989
- A uniform enzymatic method for dissociation of myocytes from hearts and stomachs of vertebratesAmerican Journal of Physiology-Heart and Circulatory Physiology, 1985
- Single cells from adult mammalian heart: Isolation procedure and preliminary electrophysiological studiesCanadian Journal of Physiology and Pharmacology, 1981
- Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patchesPflügers Archiv - European Journal of Physiology, 1981
- Active and passive electrical properties of single bullfrog atrial cells.The Journal of general physiology, 1981
- Spontaneously active cells isolated from the sino-atrial and atrio-ventricular nodes of the rabbit heart.The Japanese Journal of Physiology, 1981
- A rapid technique for the isolation and purification of adult cardiac muscle cells having respiratory control and a tolerance to calciumBiochemical and Biophysical Research Communications, 1976