Nucleotide sequence of the lig gene and primary structure of DNA ligase of Escherichia coli
- 1 July 1986
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 204 (1) , 1-7
- https://doi.org/10.1007/bf00330179
Abstract
The DNA ligase of Escherichia coli catalyses the NAD-dependent formation of phosphodiester linkages between 5′-phosphoryl and 3′-hydroxyl groups in DNA. It is essential for DNA replication and repair of damaged DNA strands. We determined the nucleotide sequence of the lig gene of Escherichia coli coding for DNA ligase and flanking regions. The coding frame of the gene was confirmed by the amino acid composition and the amino- and carboxyl-terminal amino acid sequences of the purified ligase. The ligase consists of 671 amino acid residues with a molecular weight of 73,690.Keywords
This publication has 42 references indexed in Scilit:
- New runaway-replication-plasmid cloning vectors and suppression of runaway replication by novobiocinGene, 1983
- Hyperproduction of Phosphate‐Binding Protein, phoS, and pre‐phoS Proteins in Escherichia coli Carrying a Cloned phoS GeneEuropean Journal of Biochemistry, 1983
- Relation between hen egg white lysozyme and bacteriophage T4 lysozyme: Evolutionary implicationsJournal of Molecular Biology, 1981
- Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencingJournal of Molecular Biology, 1980
- REGULATORY SEQUENCES INVOLVED IN THE PROMOTION AND TERMINATION OF RNA TRANSCRIPTIONAnnual Review of Genetics, 1979
- Empirical Predictions of Protein ConformationAnnual Review of Biochemistry, 1978
- Five Hundredfold Overproduction of DNA Ligase After Induction of a Hybrid Lambda Lysogen Constructed in VitroScience, 1977
- DNA degradation in an amber mutant of Escherichia coli K12 affecting DNA ligase and viabilityJournal of Molecular Biology, 1975
- Genetics and function of DNA ligase in Escherichia coliJournal of Molecular Biology, 1973
- A general method applicable to the search for similarities in the amino acid sequence of two proteinsJournal of Molecular Biology, 1970