SENSITIVITY OF MURINE HEMATOPOIETIC STEM-CELLS TO LECTIN CYTOTOXICITY

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  • 1 January 1976
    • journal article
    • research article
    • Vol. 4  (1) , 19-26
Abstract
The response of normal murine bone marrow and mastocytoma cells to the cytoxic effect of the 4 lectins WGA (wheat germ agglutinin), PHA (phytohemagglutinin), SBA (soybean agglutinin) and Con A (concanavalin A) was studied. The 2 types of cells apparently had similar sensitivities to each of the 4 lectins, as judged by decreased ability to develop colony growth in soft agar. They differed in their sensitivity to particular lectins. The cells were most sensitive to WGA, less sensitive to PHA and SBA and least sensitive to Con A. In further experiments, carried out on bone marrow cells, pluripotent stem cells (CFU-S) and committed stem cells (CFU-C) were equally sensitive to the cytotoxic effect of WGA. Incubation of bone marrow cells with 12 .mu.g/ml WGA at 37.degree. C for 30 min. had no effect on CFU-C; incubation for 60 min. produced a 70% decrease in CFU-C. Additional periods of incubation up to 120 min. did not further affect CFU-C, suggesting that some particular phase of the cell cycle may be sensitive to the cytotoxic effect of lectins.