Solubilized DNA‐dependent RNA polymerase from human placenta: A Mn2+‐dependent enzyme
- 16 October 1970
- journal article
- Published by Wiley in FEBS Letters
- Vol. 10 (4) , 257-260
- https://doi.org/10.1016/0014-5793(70)80642-1
Abstract
The 100-fold purified RNA polymerase activity from human placenta is completely dependent upon added DNA. The enzyme is most active at 3 mM Mn2+ in the presence of 100 mM (NH4)2SO4. Denatured DNA is a better template than native DNA. α-Amanitin completely inhibits the incorporation of 3H-UMP, while rifampicin has no influence upon the enzymatic activity.Keywords
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