Rapid identification ofCapnocytophaga isolated from septicemic patients

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Abstract
FourCapnocytophaga strains from blood cultures of immunocompromised patients with malignant disease and the type strains of threeCapnocytophaga species were examined and compared to strains representing five other genera that are hard to differentiate fromCapnocytophaga. With three rapid identification methods, negative catalase and oxidase reactions and positive ONPG assay,Capnocytophaga was easily separated fromEikenella corrodens, Actinobacillus actinomycetem-comitans, Cardiobacterium hominis, and CDC group DF-2.Haemophilus aphrophilus was excluded by leucine, valine and cystine arylamidase andα-glucosidase reactions (API ZYM). Further confirmatory reactions constituted gelatin hydrolysis, haemin requirement, and carbohydrate and esculin breakdown. Although rapid identification ofCapnocytophaga to the genus level was feasible, differentiation on a species level proved impossible.