Studies on flavinadenine dinucleotide-synthesizing enzyme in plants

Publisher Website
Google Scholar
Abstract
The occurrence of flavinadenine dinucleotide-synthesizing enzyme has been demonstrated in plants and its properties have been studied by the application of circular-paper chromatography. Fourteen plants were examined and the detailed study was confined to 1 of them, Phaseolus radiatus. Under the experimental-conditions described, maximum synthesis of flavinadenine dinucleotide was observed at pH 7.4. The Michaelis constant for flavin mononucleotide was 0.043 mM and adenosine triphosphate was 0.75 mM. Mg2+ and Zn2+ ions activated at all the concentrations studied, whereas Mn2+ ions activated at the lowest concentration, but had no effect at mM and inhibited at the highest concentration. Ni2+, Cu2+, Hg2+ and CN- ions inhibited at all the concentrations, whereas Co2+, Fe3+ and Cd2+ ions had no effect. An 83-fold purification of the enzyme was achieved by dialysis, fractionation with ammonium sulphate, adsorption and elution from alumina Cgamma-gel, and precipitation with ethanol. There was a parallel increase in flavinadenine dinucleotide content and synthesizing activity for this compound with the progress of germination.