Beet Pseudo-Yellows Virus: Purification and Serology

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Abstract
Beet pseudo-yellows virus (BPYV) has been purified from BPYV-infected Nicotiana clevelandii. Purified preparations had an A260/280nm ratio of 1.315 and contained long, flexuous rod-shaped particles approximately 12 nm wide an d1,500-1,800 nm long. Virus yield ranged from 100 to 400 .mu.g/kg of leaf tissue. An extinction coefficient of 3 (mg/ml)-1 .times. cm-1 at A260nm was used. An antiserum to BPYV was prepared that has enabled us to diagnose BPYV-infected plants by the indirect enzyme-linked immunosorbent assay (ELISA) but not the direct ELISA test. The disease, transmitted by the greenhouse whitefly (Trialeurodes vaporariorum), has been diagnosed previously only by transmission and host range tests. This is the first report of the description of the particle morphology of BPYV and the production of an antiserum that can be used for serodiagnosis.

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