Evaluation of the microplate hybridization method for rapid identification ofLegionella species
- 1 March 1990
- journal article
- research article
- Published by Springer Nature in European Journal of Clinical Microbiology & Infectious Diseases
- Vol. 9 (3) , 213-217
- https://doi.org/10.1007/bf01963841
Abstract
Rapid genetic identification of 23Legionella species was performed using microdilution plate hybridization. When an isolate appeared to be a strain of theLegionella species, its DNA was quickly extracted, labelled with photobiotin and distributed to microdilution wells where 23 different DNAs had previously been immobilized. After hybridization for 90 min at 45 °C, the microdilution well which emitted the maximum fluorescence was determined. The criterion for interpreting results of this genetic identification method is given.Keywords
This publication has 6 references indexed in Scilit:
- Fluorometric Deoxyribonucleic Acid-Deoxyribonucleic Acid Hybridization in Microdilution Wells as an Alternative to Membrane Filter Hybridization in which Radioisotopes Are Used To Determine Genetic Relatedness among Bacterial StrainsInternational Journal of Systematic and Evolutionary Microbiology, 1989
- Simple and rapid genetic identification of Legionella species with photobiotin-labeled DNA.The Journal of General and Applied Microbiology, 1988
- Evaluation of the Gen-Probe DNA probe for the detection of legionellae in cultureJournal of Clinical Microbiology, 1986
- Ten New Species of LegionellaInternational Journal of Systematic and Evolutionary Microbiology, 1985