Kinetic Study on the Oxidation of L-Proline by D-Amino-Acid Oxidase
- 1 September 1968
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 64 (3) , 371-376
- https://doi.org/10.1093/oxfordjournals.jbchem.a128904
Abstract
1. Oxidation of L-proline by D-amino-acid oxidase [EC 1.4.3.3], at high concentrations, was confirmed by polarographic and spectroscopic studies under aerobic and anaerobic conditions, respectively. 2. Under anaerobic conditions, the formation of the enzyme-substrate complex was demonstrated spectroscopically. The absorption spectrum indicated that the enzyme in the complex exists in its oxidized state. 3. The rate constant of the formation of the enzyme-substrate complex is definitely larger than that of its breakdown. 4. L-Proline behaves as an inhibitor of this oxidase in competition with D-alanine.Keywords
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