The TC10-interacting protein CIP4/2 is required for insulin-stimulated Glut4 translocation in 3T3L1 adipocytes
- 19 September 2002
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 99 (20) , 12835-12840
- https://doi.org/10.1073/pnas.202495599
Abstract
The GTPase TC10 plays a critical role in insulin-stimulated glucose transport. We report here the identification of the TC10-interacting protein CIP4/2 (Cdc42-interacting protein 4/2) as an effector in this pathway. CIP4/2 localizes to an intracellular compartment under basal conditions and translocates to the plasma membrane on insulin stimulation. Overexpression of constitutively active TC10 brings CIP4/2 to the plasma membrane, whereas overexpression of an inhibitory form of TC10 blocks the translocation of CIP4/2 produced by insulin. Overexpression of mutant forms of CIP4/2 containing an N-terminal deletion or with diminished TC10 binding inhibits insulin-stimulated Glut4 translocation. These data suggest that CIP4/2 may play an important role in insulin-stimulated glucose transport as a downstream effector of TC10.Keywords
This publication has 35 references indexed in Scilit:
- Small GTP-binding Protein TC10 Differentially Regulates Two Distinct Populations of Filamentous Actin in 3T3L1 AdipocytesMolecular Biology of the Cell, 2002
- Cloning and Functional Characterization of Related TC10 Isoforms, a Subfamily of Rho Proteins Involved in Insulin-stimulated Glucose TransportPublished by Elsevier ,2002
- Insulin-stimulated GLUT4 Translocation in Adipocytes Is Dependent upon Cortical Actin RemodelingJournal of Biological Chemistry, 2001
- Lipid raft microdomain compartmentalization of TC10 is required for insulin signaling and GLUT4 translocationThe Journal of cell biology, 2001
- Insulin-stimulated GLUT4 translocation requires the CAP-dependent activation of TC10Nature, 2001
- SynipMolecular Cell, 1999
- Identification of Inducible Genes at the Early Stage of Adipocyte Differentiation of 3T3-L1 CellsBiochemical and Biophysical Research Communications, 1999
- PTG, a Protein Phosphatase 1-Binding Protein with a Role in Glycogen MetabolismScience, 1997
- Wiskott–Aldrich Syndrome Protein, a Novel Effector for the GTPase CDC42Hs, Is Implicated in Actin PolymerizationCell, 1996
- Basic local alignment search toolJournal of Molecular Biology, 1990