A phosphatidylinositol-specific phospholipase C from Cytophaga: production, purification, and properties
- 1 December 1992
- journal article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 38 (12) , 1334-1337
- https://doi.org/10.1139/m92-220
Abstract
An extracellular phosphatidylinositol-specific phospholipase C was isolated from a Cytophaga species. The growing conditions were optimized for the production of the enzyme and a purification scheme was developed. The enzyme is small and is not dependent on metal ions for activity. It has a rather low apparent Michaelis constant, 0.2 mM, for phosphatidylinositol and is not stimulated by deoxycholate or extra phospholipid. It releases alkaline phosphatase from rat kidney and cholinesterase from plaice muscle tissue but not acetylcholinesterase from intact rat or pig erythrocytes. Key words: phosphatidylinositol specific, phospholipase, Cytophaga.Keywords
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