Temporal and spatial expression of two isoforms of theDutt1/Robo1gene in mouse development
Open Access
- 21 June 2002
- journal article
- Published by Wiley in FEBS Letters
- Vol. 523 (1-3) , 12-16
- https://doi.org/10.1016/s0014-5793(02)02904-6
Abstract
The mammalian homologue of the Drosophila axonal guidance receptor roundabout is expressed in a wide range of tissues. Here we show that alternative splicing of the Dutt1/Robo1 gene results in two mRNA transcripts with different signal peptides, which are differentially expressed throughout mouse embryogenesis. Since mice with a targeted deletion in the Dutt1/Robo1 gene have abnormal lung pathology, immunohistochemistry was used to identify the cellular expression pattern of Dutt1/Robo1 during lung development. Dutt1/Robo1 expression was widespread and diffuse in the lung at embryonic day 17.5 but became increasingly localised to the bronchial epithelium in newborn and adult mice.Keywords
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