Liquid Chromatographic Analysis of Bile Acid Methyl Esters
- 1 May 1976
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 9 (5) , 429-438
- https://doi.org/10.1080/00032717608059106
Abstract
Methods have been developed for the qualitative and quantitative analysis of hydroxy and keto derivatives of methyl cholanates utilizing a ¼″ by 1′ μPorasil column, various mixtures of hexane and ethyl acetate and a refractive index detector. The system has been calibrated for some of the methyl dihydroxycholanates using 3α;,7α-dihydroxy-12-oxocholanate as an internal standard and applied to the analysis of the mixtures of diols resulting from the reduction of methyl 3,12-dioxocholanate with NaBH4 and with Raney nickel.Keywords
This publication has 1 reference indexed in Scilit:
- 3β, 12β-Dihydroxycholanic Acid*The Journal of Organic Chemistry, 1965