Histochemical and immunohistochemical properties of the CRF-Granules and other „Gomori-positive” substances of the rat

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Abstract
In adrenalectomized rats, histochemical and immunohistochemical properties of the following secretion products have been investigated: CRF-granules in the outer layer of the median eminence; neurosecretory material (NSM) in the supraoptico-hypophysial system of the hypothalamus; secretory granules in the TSH-cells of the anterior lobe of the hypophysis; secretory granules in the ependymal cells of the subcommissural organ (SCO); β-cell-granules in the islets of Langerhans in the pancreas. All these substances are characterized by their stainability with the so-called “Gomorimethod”. The experiments have included studies into: the extractability of the substances by various solvents; the digestability of the substances by pepsin or trypsin; their histochemically detectable content of disulfide groups, arginine and periodic acid-Schiff (PAS) reactive carbohydrates; their reaction with porcine-neurophysin-II-antibodies. All substances exhibited a positive reaction for disulfide groups. Based on their solutility properties, their resistance to pepsin or trypsin, their respective content of PAS-reactive carbohydrates and their failure to react with anti-neurophysin serum the “Gomori-positive” granules in TSH-, SCO- and pancreatic β-cells can be distinguished from one another and from CRF- and neurosecretory granules. In contrast, CRF-granules and NSM showed identical properties. Taking into consideration data from the biochemical and histochemical literature, the present findings suggest that CRF-granules and NSM consist of closely related biochemical substances.

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