K ANTIGEN DETERMINATION OF ESCHERICHIA COLI BY COUNTER-CURRENT IMMUNOELECTROPHORESIS (CIE)

15 August 2009

journal article
research article
Published by Wiley in Acta Pathologica Microbiologica Scandinavica Section B Microbiology

Vol. 85B (1) , 103-107
https://doi.org/10.1111/j.1699-0463.1977.tb01680.x

Abstract

The application of counter-current immunoelectrophoresis (CIE) for determination of E. coli acidic polysaccharide K antigen is described. The most appropriate dilutions of antigens and [rabbit] antisera were established after examination of 6 different K antigens with homologous OK antisera. All test strains for acidic polysaccharide K antigens, i.e., K1 to K57, K62, K74, K82, K83, K84, K92, K93, K94, K95, K96, K97, K98 and K100, were examined; OK antisera suitable for the CIE test were selected. The following reciprocal cross-reactions were found: K2 and K62; K7 and K56; K12 and K82; K13, K20 and K23; K18 and K22; K16 and K97; and K53 and K93. The CIE method is now used as a routine test in this laboratory.

Keywords

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