Antibody responsiveness to the enteropathogenic Escherichia coli bundle-forming pilus subunit A in lactating mothers and asymptomatic children
- 10 July 2002
- journal article
- Published by Springer Nature in European Journal of Pediatrics
- Vol. 161 (9) , 471-474
- https://doi.org/10.1007/s00431-002-0995-8
Abstract
The objective of this study was to examine the antibody responsiveness to the bundle-forming pilus subunit A of enteropathogenic Escherichia coli in human colostrum (n=36) and serum (n=90) of paediatric ambulatory patients, 3–24 months of age. Affinity purified recombinant bundle-forming pilus subunit A was used in immunoblot analysis to detect antigen-specific serum immunoglobulins G and A, and colostrum immunoglobulin A. Circulating immunoglobulin G antibody activity to the bundle-forming pilus subunit A was readily detected in all children. The frequency of immunoglobulin A antibody activity to the bundle-forming pilus subunit A in serum was 83.3%. Immunoglobulin A antibody activity to the bundle-forming pilus subunit A was detected in 75% of colostrum samples. The high frequency of immunoglobulin A antibody activity to the bundle-forming pilus subunit A observed in serum reveals a predominance of typical enteropathogenic Escherichia coli priming/infection in the study population. Conclusion: these findings demonstrate that the bundle-forming pilus subunit A is immunogenic, for both immunoglobulins G and A, in both pregnant/lactating women and asymptomatic children.Keywords
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