Abstract
Since few oocysts sporulate in water, it appears that bacteria inhibit sporulation. This bacterial effect may explain why natural infections tend to be light. Oocysts showed marked resistance to the effects of rather high concentrations of sodium chloride. Sporulation was inhibited less by dilute acids than bases. No oocysts sporulated at moderately low temperatures, although oocysts remained viable for considerable periods of time at these low temperatures. Little sporulation occurred at 35[degree]C and none at 37[degree]C or higher. No oocysts were infective after drying at room temperature for 4 days. Reduced sporulation was observed under anaerobic conditions.
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