Nucleosides and nucleotides part 24 Investigation of ribo‐ and deoxyribonucleosides and ‐nucleotides by fast‐ atom‐bombardment mass spectrometry

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Abstract
The fast‐atom‐bombardment mass spectra (FAB‐MS) of a series of ribo‐ and deoxyribonucleosides and ‐nucleotides were examined in both positive‐ion and negative‐ion modes. Behaviour of cytosine analogs having unnatural bases (2(1H)‐pyrimidone, 2(1H)‐pyridinone, and 4‐amino‐2(1H)‐pyridinone) was similar to that of compounds having naturally occurring bases. Nucleotides protected by dimethoxytrityl and p‐chlorophenyl groups were also investigated using this technique. Use of negative‐ion mode reduces interference from positive counter‐ions (e.g., Na+) and allows rapid sequence determination of simple di‐ and trinucleotides.