Mice cloned from olfactory sensory neurons
Top Cited Papers
- 15 February 2004
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 428 (6978) , 44-49
- https://doi.org/10.1038/nature02375
Abstract
Cloning by nuclear transplantation has been successfully carried out in various mammals, including mice. Until now mice have not been cloned from post-mitotic cells such as neurons. Here, we have generated fertile mouse clones derived by transferring the nuclei of post-mitotic, olfactory sensory neurons into oocytes. These results indicate that the genome of a post-mitotic, terminally differentiated neuron can re-enter the cell cycle and be reprogrammed to a state of totipotency after nuclear transfer. Moreover, the pattern of odorant receptor gene expression and the organization of odorant receptor genes in cloned mice was indistinguishable from wild-type animals, indicating that irreversible changes to the DNA of olfactory neurons do not accompany receptor gene choice.Keywords
This publication has 40 references indexed in Scilit:
- Negative Feedback Regulation Ensures the One Receptor-One Olfactory Neuron Rule in MouseScience, 2003
- A monoclonal mouse?Nature, 2002
- Nuclear Cloning and Epigenetic Reprogramming of the GenomeScience, 2001
- Monoallelic expresion of the odourant receptor gene and axonal projection of olfactory sensory neuronesGenes to Cells, 2001
- Genetic Ablation and Restoration of the Olfactory Topographic MapCell, 2000
- Generation of mice from wild-type and targeted ES cells by nuclear cloningNature Genetics, 2000
- YAC transgene-mediated olfactory receptor gene choiceDevelopmental Dynamics, 2000
- Rearranging Views on Neurogenesis: Neuronal Death in the Absence of DNA End-Joining ProteinsNeuron, 1999
- An immunochemical, ultrastructural, and developmental characterization of the horizontal basal cells of rat olfactory epitheliumJournal of Comparative Neurology, 1995
- A novel multigene family may encode odorant receptors: A molecular basis for odor recognitionCell, 1991