Effects of Histamine on Protein and Glycoprotein Production of Isolated Pig Gastric Mucosal Cells

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Abstract
The production of glycoprotein and protein by isolated pig gastric non-parietal cells was measured by incorporation of N-acetyl-[14C]-D-glucosamine ([14C]GlcNAc) and [3H]-L-leucine ([3H]Leu), respectively, into acid insoluble material (AIM). Histamine enhanced incorporation of the tracers into cellular and released AIM in a concentration-dependent manner. The H2 receptor antagonist ranitidine completely blocked the effects of histamine (100 .mu.mol/l) on [3H]Leu incorporation into cellular and released AIM (IC50 37 and 32 .mu.mol/l, respectively) but had no inhibitory effect on the 16,16-dimethylprostaglandin-E2- and forskolin-stimulated tracer incorporation. The H1 receptor antagonist mepyramine did not inhibit the histamine effect. We conclude that histamine is a stimulant of protein, via H2 receptors, and glycoprotein production of isolated pig gastric non-parietal cells.

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