Determination of Parishin, Parishins B and C in Traditional Chinese Medicinal Preparations by High Performance Liquid Chromatography

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Abstract
High performance liquid chromatographic methods were established for the determination of parishin, parishins B and C in three kinds of the traditional Chinese medicinal prescriptions, including Gastrodiae Rhizoma: Shuen-Feng-Iun-Chi-San, Ban-Shia-Bai-Ju-Tian-Ma-Tang and Tsan-Siang-Tian-Ma-Tang. The samples were analyzed with an Inertsil ODS-2 reversed phase column by gradient elution with varied 0.1% (v/v) phosphoric acid-acetonitrile as mobile phases and detected at UV 222 nm. Ethoxybenzamide was used as an internal standard. Regression equations were derived showing linear relationships (correlation coefficients: 0.9976–0.9999) between the peak area ratios of each marker (parishin, parishins B and C) to ethoxybenzamide and concentration. The recoveries of parishin, parishins B and C from the Chinese medicinal preparations were 102.0–104.1%, 102.9–105.0% and 103.2–104.0%, respectively. The relative standard deviations of three marker constituents ranged between 0.90–2.47% (intraday) and 1.05–4.41% (interday). The contents of parishin, parishins B and C measured 1.58–2.85 mg/g, 1.58–3.25 mg/g and 1.45–1.72 mg/g, respectively.

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