A plate technique is described for obtaining clones of T. vaginalis. Under anaerobic conditions organisms developed into colonies ranging from 0.2 to 2.0 mm in size in about 5 days. Macroscopic and microscopic colony growth over a period of days were described. Certain limitations of the procedure were investigated and clarified. Clone growth occurred in medium containing ionagar within the range of 0.35 arid 0.55%. Prolonged exposure of organisms to oxygen and/or 25[degree]C proved detrimental to clone development. Potential uses of the procedure were discussed.