Theory and Use of Centrifugal Partition Chromatography

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Abstract
Centrifugal Partition Chromatography (CPC) is a variant of countercurrent chromatography (CCC). As in CCC, two immiscible liquids are used. The first liquid is the stationary phase, the second is the mobile phase. The liquid stationary phase is held in channels engraved in seveal polychlorotrifluoroethylene (PCTFE) plates. One hundred channels are engraved on each PCTFE plate. Four PCTFE plates are assembled together in a cartridge. Up to 12 cartridges (4800 channels) can be loaded in the rotor of a centrifuge. The centrifugal field, generated by the spinning rotor, holds the stationary phase sufficiently that a mobile phase can be pumped through in (Figure 1). The system is analyzed in detail.