Mistranslation in cells infected with the bacteriophage MS2: Direct evidence of Lys for Asn substitution
- 1 November 1980
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 180 (2) , 275-281
- https://doi.org/10.1007/bf00425839
Abstract
The coat protein of the bacteriophage MS2 was found to show an increased level of charge heterogeneity when synthesized in Escherichia coli starved for Asn or Lys. No such increase was found when the host was starved for Arg, His, Ile or Pro. This is the pattern predicted by “two-out-of-three” codon misreading in the coat protein gene. In the case of Asn starvation, direct measurements of the relative incorporation of Lys demonstrate that the observed charge heterogeneity is the result of mistranslation. Asn starvation increased the error frequency in coat protein to over 0.3 mistake per asparagine codon. The small amount of charge heterogeneity seen in unstarved cells seems also to be the result of misreading Asn codons.This publication has 32 references indexed in Scilit:
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