Substitution of an invariant nucleotide at the base of the highly conserved ‘530–loop’ of 15S rRNA causes suppression of yeast mitochondrial ochre mutations

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Abstract
We have determined the nucleotide sequence alteration in the 15S rRNA gene of a Saccharomyces cerevisae strain carrying the previously described mitochondrial ochre suppressor, MSU1. The suppressor contains an A residue at position 633 of the yeast mitochondrial sequence, in place of the wild-type G. This position, located in the higly conserved region forming the stem of the ''530-loop'', corresponds to G517 of the Escherichia coli 16S rRNA and is occupied by G in all other known small rRNA sequences. This finding strongly supports the previous conclusions of others that the 530-loop region plays an important role in enhancing translational accuracy.