Molecular Detection of Histoplasma capsulatum var. capsulatum in Human Clinical Samples
Open Access
- 1 April 2003
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 41 (4) , 1753-1755
- https://doi.org/10.1128/jcm.41.4.1753-1755.2003
Abstract
We developed a seminested PCR for the diagnosis of histoplasmosis that amplifies a portion of the Histoplasma capsulatum H antigen gene. This assay is highly sensitive and specific, being able to detect genomic material corresponding to less than 10 yeast cells without cross-reaction against other bacterial or fungal pathogens.Keywords
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