The Molar Absorptivity of Horseradish Peroxidase.

Abstract
The absorption coefficient for the Soret band of horseradish peroxidase (E.C. 1.11.1.7) has been variously given as .epsilon.mM = 91-107 and this uncertainty has hampered kinetic and stoichiometric analyses. The enzyme follows Beer''s law, contrary to some reports. Lyophilization or freezing (-18.degree. C) of buffered solutions sometimes causes losses of activity and alters the spectrum.

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