Characterization with 3H-haloperidol of the dopamine receptor in the rat kidney particulate preparation.

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Abstract
The dopamine receptor of rat kidney particulate preparation was identified and characterized by the use of 3H-haloperidol, a neuroleptic, binding. Binding of 3H-haloperidol to the kidney particulate preparation was slow and saturable. The Kd were 0.41 and 5.88 nM, respectively, according to the model of 2 classes of independent binding sites. Maximal binding of high affinity site was obtained with 166 f[fumato]mole/mg protein which was about 40% of the total receptor density. A wide variety of neuroleptics at specifically low concentrations in nanomolar range inhibited the 3H-haloperidol binding. There was an excellent correlation between the affinity of numerous neuroleptics for the kidney particulate preparation and that for the brain striatum.