Enzyme immunoassay of neocarzinostatin using .BETA.-galactosidase as label.

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Abstract
A novel convenient procedure was introduced for enzyme labeling of neocarzinostatin (NCS) with .beta.-D-galactosidase using a new hetero-bifunctional reagent N-(.gamma.-maleimidobutyryloxy)succinimide (GMBS). With the enzyme labeled NCS and a rabbit anti-NCS serum which was elicited in a rabbit immunized with NCS in complete Freund''s adjuvant, a highly sensitive enzyme immunoassay which can quantify a femtomol order of NCS was developed. Accuracy and precision of the assay and a comparison with the immunodiffusion method were studied with satisfactory results. This enzyme immunoassay of NCS was applicable to monitoring serum NCS levels of patients in clinical treatment of the antitumor agent.