Genome Size and Deoxyribonucleic Acid Base Composition of Thermoplasma acidophilum

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Abstract
The deoxyribonucleic acid base composition of two strains of Thermoplasma acidophilum including the type strain 122-1B2 was determined by buoyant density and thermal denaturation temperature. The guanine plus cytosine content of the two strains examined was found by both methods to be about 46%. This result is strikingly at variance with the significantly lower values, about 25%, reported by the group of workers who first described this organism. The genome size, as determined by the renaturation method of Wetmur and Davidson, was found to be about 109 daltons. This is identical with the genome size of members of the family Acholeplasmataceae, order Mycoplasmatales, within which order T. acidophilum has been tentatively classified.

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