Identification of 'Campylobacter upsaliensis' and other catalase-negative campylobacters from paediatric blood cultures by numerical analysis of electrophoretic protein patterns
Open Access
- 1 April 1989
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 58 (2-3) , 145-150
- https://doi.org/10.1016/0378-1097(89)90029-3
Abstract
Twenty-one strains of catalase-negative campylobacters from paediatric blood cultures were characterized by one-dimensional SDS-PAGE of cellular proteins. A further 11 Campylobacter strains were included for reference purposes. The partial protein patterns were used as the basis of a numerical analysis, which showed that 17 hippurate-negative strains had a high similarity to ‘C. upsaliensis’ (r ⩾ 0.82) irrespective of their geographical location, and that three hippurate-positive isolates had a high similarity (r ⩾ 0.87) to C. jejuni subsp. jejuni. One hippurate-positive CNW strain was not identified. The analysis of SDS-PAGE protein patterns proved an excellent method of characterizing these thermophilic campylobacter as they were difficult to identify by traditional methods.Keywords
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