Use of the enzyme-linked immunosorbent assay for detection of antibodies toChlamydia trachomatis

Abstract
An enzyme-linked immunosorbent assay (ELISA) using a soluble antigen prepared from the D strain ofChlamydia trachomatis was used for titration of IgG antibodies toChlamydia trachomatis in 153 sera from 126 patients with non-specific genital infection and from 27 healthy subjects. The results were compared to those obtained with the micro-immunofluorescence method and were in complete agreement in 143 of the 153 sera. The ELISA proved to be reproducible and as sensitive as the micro-immunofluorescence method.