Insolubilized D-Amino Acid Oxidase: Properties and Potential Use
- 1 August 1972
- journal article
- research article
- Published by Taylor & Francis in Separation Science
- Vol. 7 (4) , 403-407
- https://doi.org/10.1080/00372367208055582
Abstract
D-Amino acid oxidase has been amide-linked to ω-aminoalkyl agarose particles. The insolubilized enzyme has characteristics similar to the soluble form with respect to stability, pH optimum, affinity for flavin adenine dinucleotide, and general substrate specificity, although smaller Michaelis constants were found for some substrates. The potential application for the separation of the natural L-amino acid isomers from racemates has been demonstrated.Keywords
This publication has 10 references indexed in Scilit:
- Purificationof Flavin Mononucleotide-dependent Enzymes by Column Chromatography on Flavin Phosphate Cellulose CompoundsPublished by Elsevier ,2021
- Affinity ChromatographyAnnual Review of Biochemistry, 1971
- Release of α-imino acid as primary product in d-amino-acid oxidase reactionBiochimica et Biophysica Acta (BBA) - Enzymology, 1970
- Carrier-bound proteins: Properties of peroxidase bound to insoluble carboxymethylcellulose particlesArchives of Biochemistry and Biophysics, 1969
- D-Amino Acid OxidasePublished by Elsevier ,1969
- Single-step purification of avidin from egg white by affinity chromatography on biocytin-Sepharose columnsBiochemical and Biophysical Research Communications, 1968
- Water-Insoluble Derivatives of Enzymes, Antigens, and AntibodiesAnnual Review of Biochemistry, 1966
- Elements of Inorganic Chemistry. Robert A. Plane and Ronald E. Hester. Benjamin, New York, 1965. xvi + 188 pp. Illus. Paper, $3.95; cloth, $8Science, 1965
- d-amino acid oxidase I. Dissociation and recombination of the holoenzymeBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1965
- d-amino acid oxidase II. Specificity, competitive inhibition and reaction sequenceBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1965