Clonal differences within M-types of the group A streptococcus revealed by pulsed field gel electrophoresis
- 1 February 1992
- journal article
- research article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 91 (1) , 85-89
- https://doi.org/10.1016/0378-1097(92)90567-8
Abstract
Digestion of chromosomal DNA with the rare cutting restriction enzyme Sfi I in association with pulsed field gel electrophoresis was used to observe restriction fragment length polymorphisms (RFLP) among isolates of group A Streptococcus. Streptococci examined included isolates belonging to the same M-type (epidemiologically related and unrelated), and isolates from other M-types. RFLP patterns were quite distinct between all serotypes tested. More importantly, isolates from within a serotype could be differentiated by this technique.Keywords
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