Surface Localization, Regulation, and Biologic Properties of the 96-kDa Alcohol/Aldehyde Dehydrogenase (EhADH2) of Pathogenic Entamoeba histolytica
Open Access
- 1 January 1996
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Infectious Diseases
- Vol. 173 (1) , 226-231
- https://doi.org/10.1093/infdis/173.1.226
Abstract
The 96-kDa surface antigen of Entamoeba histolytica was demonstrated through extensiveimmunologic evaluation with monoclonal and monospecific antibodies to be identical to or an isoform of the amebic alcohol/aldehyde dehydrogenase (EhADH2). EhADH2 was secreted, excreted, or shed into the culture medium in quantities commensurate with amebic growth when studied in a novel culture system. Of importance, using RNase protection assays, specific mRNA coding for the EhADH2 gene product(s) was up-regulated by treatment of viable trophozoites with the enzyme substrate ethanol. These data provide insight into the biology of this enzyme and its regulation by appropriate stressors.Keywords
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