AN ENTEROTOXIN PRODUCED BY CLOSTRIDIUM PERFRINGENS TYPE D. PURIFICATION BY AFFINITY CHROMATOGRAPHY

1 September 1976

journal article
research article
Published by Wiley in Acta Pathologica Microbiologica Scandinavica Section B Microbiology

Vol. 84B (6) , 414-420
https://doi.org/10.1111/j.1699-0463.1976.tb01960.x

Abstract

Clostridium perfringens type D 9867 produced enterotoxin immunologically identical to that produced by types A and C which are responsible for C. perfringens food poisoning. Enterotoxin from type C 5386 and type D 9867 was produced in Duncan og Strong sporulation medium (DS medium). The supernatant fluid from DS-cultures was used for purification of the enterotoxin by affinity chromatography on a monospecific anti-enterotoxin-coupled CNBr activated-Sepharose 4B and activated CH-Sepharose 4B column. The enterotoxin purified by this one-step procedure proved to be of a purity comparable to that obtained by conventional methods, and possessed lethal activity in mice.

Keywords

This publication has 29 references indexed in Scilit:

EXPERIMENTAL DIARRHEA IN CYNOMOLGUS MONKEYS BY ORAL ADMINISTRATION WITH CLOSTRIDIUM PERFRINGENS TYPE A VIABLE CELLS OR ENTEROTOXIN
Japanese Journal of Medical Science and Biology, 1975
Distribution of Enterotoxin-producing Strains of Clostridium perfringens Type A in Human Beings, Foods and Soils
Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi), 1975
Purification and characteristics of the enterotoxin of Clostridium perfringens type A
Canadian Journal of Microbiology, 1971
Disc Electrophoresis
The American Biology Teacher, 1966
DISC ELECTROPHORESIS – II METHOD AND APPLICATION TO HUMAN SERUM PROTEINS*
Annals of the New York Academy of Sciences, 1964
The types of Clostridium perfringens
The Journal of Pathology and Bacteriology, 1964
Routine typing of Clostridium welchii
Epidemiology and Infection, 1953
PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT
Journal of Biological Chemistry, 1951
Enteritis Necroticans due to Clostridium Welchii Type F
BMJ, 1949