Lipid Quantification of Freshwater Invertebrates: Method Modification for Microquantitation

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Abstract
To facilitate determination of lipid content of freshwater invertebrates for bioaccumulation studies, the chloroform–methanol extraction procedure of Bligh and Dyer was modified for microquantitation. Following extraction, lipids were quantified by use of a micro-balance. Recoveries of representative neutral and polar lipids averaged 88.3% with a mean coefficient of variation of 8.6%. Tests with freshwater benthic and pelagic invertebrates demonstrated the applicability of the procedure to samples as small as several milligrams wet weight with a mean coefficient of variation of 11.6%.