Isolation and properties of the pituitary gonadotropin from silver carp (Hypophthalmichthys molitrix)
- 1 February 1988
- journal article
- research article
- Published by Wiley in International Journal of Peptide and Protein Research
- Vol. 31 (2) , 150-156
- https://doi.org/10.1111/j.1399-3011.1988.tb00017.x
Abstract
The gonadotropin of silver carp (scGTH) was purified from the pituitary glands in a yield of 8.4 mg g through extraction with 6% ammonium acetate (pH 5.1)–40% ethanol followed by 80% ethanol precipitation. DEAE-cellulose chromatography and gel filtration. The molecular weight of scGTH is about 32 000 as estimated by gel filtration. Leucine and serine were the two N-termini of scGTH. The amino acid composition of scGTH is rich in aspartic acid asparagine, glutamic acid/glutamine, valine, proline and half cystine. Its carbohydrate content is 22.2%. Biologically, scGTH significantly stimulated the steroidogenesis in carp testis and ovary in vitro and induced the ovulation in loaches. It also stimulated the 32P-phosphate uptake into 1-day-old chicken testis. Immunologically. scGTH could cross-react with the anti-serum against cGTH and vice versa. In both cases parallel displacement curves were displayed.Keywords
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