Nuclear translocation of PKCζ during ischemia and its inhibition by wortmannin, an inhibitor of phosphatidylinositol 3‐kinase

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Abstract
Protein kinase Cζ (PKCζ), a member of the atypical PKC subgroup, is insensitive to Ca2+, diacylglycerol, and phorbol esters, but is activated by phospholipids such as phosphatidylinositol‐3,4,5‐triphosphate, a product of phosphatidylinositol 3‐kinase (PI3‐kinase). Here we show that PKCζ translocates from the cytosol to the 1000×g pellet (nuclear‐myofibrillar) fraction during ischemia for 40 min in Langendorff‐perfused rat hearts. In addition, immunohistochemical observation shows that ischemia induces the translocation of PKCζ to the nucleus. The nuclear translocation during ischemia is inhibited in a dose‐dependent manner by wortmannin (10−9–10−7 M), an inhibitor of PI3‐kinase.