INCREASED MEMBRANE BINDING OF ERYTHROCYTE CATALASE IN HEREDITARY SPHEROCYTOSIS AND IN METABOLICALLY STRESSED NORMAL CELLS

  • 1 January 1977
    • journal article
    • research article
    • Vol. 49  (1) , 113-123
Abstract
Normal red blood cell (RBC) membranes were compared with (1) RBC membranes from 6 patients with hereditary spherocytosis (HS), (2) normal membranes after hemolysis of the RBC in the presence of Ca or (3) normal membranes after incubation of RBC for 24 h in phosphate-buffered saline containing Ca without added glucose. When compared with normal controls, polyacrylamide gel electrophoresis with sodium dodecyl sulfate (PAGE SDS) of all 3 preparations showed an increase in membrane binding of globin and protein band 4.5 (60,000 MW). In an attempt to identify band 4.5, 14 enzymes were assayed in the RBC membranes. Of these, catalase and lactate dehydrogenase were increased in membranes from HS RBC and from normal cells exposed to Ca. Only catalase was present in sufficient quantity and had the correct subunit molecular weight on PAGE SDS and Ca-dependent membrane binding to account for an appreciable portion of 4.5. Catalase was further identified with a component of band 4.5 by double immunodiffusion using a specific anti-catalase antibody.

This publication has 6 references indexed in Scilit: