Comparison of the Phenotyping Methods ID 32E and VITEK 2 Compact GN with 16S rRNA Gene Sequencing for the Identification of Enterobacter sakazakii

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Abstract
A total of 34 isolates (28 Enterobacter sakazakii and 6 Enterobacteriaceae ) from infant formulae, milk powder, and the production environment of milk powder factories were identified using ID 32E and VITEK 2 compact GN systems (bioMérieux, France). The ID 32E version 3.0 and VITEK 2 compact GN version 01.01b correctly identified 100% (28) of the Enterobacter sakazakii isolates tested, whereas the previous software version 2.0 for ID 32E showed only 71.4% correct results. None of the non- E. sakazakii isolates tested were misidentified as E. sakazakii with either of the identification systems used.